Fig 8. Population behavior of split drive components Rescue^tko and Cas9/Cleaver alone and together as a complete split^50cM ClvR V2 element.

Split^50cM ClvR Rescue/Cargo/gRNAs spreads to very high frequency at the same time as Cas9 frequency undergoes a steep decline. Behavior of Rescue-only (A) and Cas9/Cleaver-only (B) behavior in a WT (w¹¹¹⁸) background. (C) Genotype frequencies of Rescue^tko (solid lines) and Cas9/Cleaver (dashed lines) when introduced together as a complete split^50cM ClvR V2 element, in four replicates (red, green, orange, and yellow). Predicted drive behavior from a model in which Rescue^tko and Cleaver have additive fitness costs of 6.5% and 7.5%, respectively, are shown a blue lines (see methods for details). (D) Drive populations from (C) to which a 50% WT addition was made following generation 15.