Table 1.

Overview of 102 essential gene silencing experiments

Target gene	Cultures#	Stable kd *	Stable miniCR	Maximum kd (%)§	Revertants&	Strong miniCR$
slaB+	52	30	SB-2, SB-3, SB-3x2, SB-3x6	75	22	SB-123, SB-23, SB-3x6 (colony)
aif5A+	11	6	aIF5A-II	40	5	aIF5A-I
cdvABC	23	10	CA-2	50	13	CA-1, CA-3, CA-123
smAP2	16	13	SM2	60	3	SM2 (colony)

^#Cultures: Number of independently transformed cultures carrying a miniCRISPR vector; *Stable Kd: Number of cultures with measurable and stable knockdown of the essential gene; ^§Maxmimum kd: maximum knockdown observed in stably silenced cultures expressed as % mRNA lost compared to Ctrl; ^&Revertants: Number of cultures reverted to wild type owing to strong miniCRs; ^$Strong miniCR: miniCR conveying strong silencing leading to their instability; + Data pooled from published studies (ref. [31,32]) and from freshly transformed cultures. Colony = constructs changed after recovery from a single colony