Skip to main content
. 2021 Mar 1;17(3):e1009368. doi: 10.1371/journal.ppat.1009368

Fig 3. The Asn261Lys polymorphism in Pikh-1 extends association to AVR-PikE and AVR-PikA in planta.

Fig 3

Co-immunoprecipitation of full length Pikp-1 and Pikh-1 with AVR-Pik variants. N-terminally 4xMyc tagged AVR-Pik effectors were transiently co-expressed with Pikp-1:6xHis3xFLAG (left) or Pikh-1:6xHis3xFLAG (right) in N. benthamiana. Immunoprecipitates (IPs) obtained with M2 anti-FLAG resin and total protein extracts were probed with appropriate antisera. Each experiment was repeated at least three times, with similar results. The asterisks mark the Pik-1 band. Total protein extracts were coloured with Ponceau Staining (PS).