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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Mol Carcinog. 2021 Feb 5;60(3):213–223. doi: 10.1002/mc.23285

Figure 2. Effects of 2HF and RLIP-targeting agents on protein expression in the tumor tissues of MMTV-neu mice.

Figure 2

Sixty microgram protein of tumor tissues excised from MMTV-neu mice that received 28 weeks of control treatments (corn oil [C1], CAS [C2], and PIS [C3]) or experimental treatments (2HF [T1], RAS [T2], Rab [T3], and 2HF+RAS+Rab [T4]) were analyzed for changes in RLIP, pAkt, Bax, Bcl2, and CDK1 expression. β-actin was used as a loading control. The experiment was performed three times and the image shown is representative of one experiment. The numbers below the blots represent the fold-change in protein expression levels in the tumor tissues of mice in the experimental treatment groups compared to the control treatment groups, as determined by densitometry. Bar diagram shows the quantification of respective Western blots. Dotted line represents no significant change as observed with respective controls.