. Author manuscript; available in PMC: 2021 Mar 11.

Published in final edited form as: J Vis Exp. 2020 Jan 7;(155):10.3791/60647. doi: 10.3791/60647

Table 3:

Oligonucleotide sequences used in this protocol.

Lipid based barcoding Oligonucleotides
Anchor LMO	5’-TGGAATTCTCGGGTGCCAAGGGTAACGATCCAGCTGTCACT-Lipid-3’
Co-Anchor LMO	5’-Lipid-AGTGACAGCTGGATCGTTAC-3’
Barcode Oligo	5’-CCTTGGCACCCGAGAATTCCANNNNNNNNA30–3’
Lipid barcoding Additive Primer	5’-CTTGGCACCCGAGAATTCC-3’
RPIX Primer	5’-CAAGCAGAAGACGGCATACGAGATNNNNNNGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA-3’
Universal Adapter Primer	5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’
Antibody based barcoding Oligonucleotides
Antibody barcoding oligo	5’-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNNNNBAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA^A^A-3’
HTO additive Primer	5’-GTGACTGGAGTTCAGACGTGTGCTC-3’
ADT additive Primer	5’-CCTTGGCACCCGAGAATTCC-3’
P5-smart-pcr hybrid oligo	5’-AATGATACGGCGACCACCGAGATCTACACGCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGT^A^C-3’

N = Barcode or index sequence;

= Phosphorothioate bond