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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Mol Cancer Ther. 2021 Mar;20(3):577–588. doi: 10.1158/1535-7163.MCT-20-0446

Figure 5. Generation and phenotype of B7-H3 CAR T cells.

Figure 5

Schematic illustration of the B7-H3 CAR construct consisting of anti-human B7-H3 scFv 376.96, CD28 costimulatory domain, and CD3ζ signaling domain (A). The time course of the number of expanded transduced and NT cells (B), as well as the percentage of B7-H3 CAR T cells out of the total CD3+ cells on day 8 in transduced T cells expanded from 3 individual donors are shown (C). Flow cytometry analysis of B7-H3 CAR T cells and NT cells for CD4+ and CD8+ T cells (D) and mean ± SD (%) of CD4+ and CD8+ CAR T cells are shown (E), in addition to the flow cytometry analysis for CD45RA+CD62L+ memory-CAR T cells (F) and mean ± SD (%) CD45RA+CD62L+ CAR T cells (G) are shown. n.s., not significant.