Fig. 1.

3-Methyladenine (3-MA) promoted the inhibitory effect of sorafenib on HepG2 cell proliferation. (a) HepG2 cells were treated with gradient concentrations of sorafenib (2.5, 5, 10, 20 µM) for 12, 24 and 48 h, whereas cells treated with isopyknic DMSO were used as controls. (b) Four groups of HpeG2 cells were respectively treated with 15 µM sorafenib, 5 mM 3-MA, 15 µM sorafenib + 5 mM 3-MA and DMSO for 24 h. HepG2 cell viability was detected by cell counting kit 8. The results were obtained from at least triplicate independent experiments. Values were shown as mean ± SEM (n = 5), *P value < 0.05, **P value < 0.01. DMSO, dimethyl sulfoxide.