. 2021 Mar 11;10:e62046. doi: 10.7554/eLife.62046

Appendix 1—table 1. Sequences of DNA oligonucleotides used to construct substrates for FRET scheme 1.

Substrate	Oligo name	Sequence
Double flap	17e	5'-	TCGCAGCCGYCAATATg
	T2b	3'-BT₁₀-	AGCGTCGGCAGTTATAGATATAGCTTCGGAACAC −5'
	D18c_2		taCTATATCGAAGCCTTGTG −3'
Mixed flap	17e	5'-	TCGCAGCCGYCAATATG
	T1b	3'-BT₁₀-	AGCGTCGGCAGTTATACATATAGCTTCGGAACAC −5'
	D18b_2		taGTATATCGAAGCCTTGTG −3'
Downstream flap	17e	5'-	TCGCAGCCGYCAATATG
	T1b	3'-BT₁₀-	AGCGTCGGCAGTTATACATATAGCTTCGGAACAC −5'
	D18c_2		tacTATATCGAAGCCTTGTG −3'
Nick	17e	5'-	TCGCAGCCGYCAATATG
	T1b	3'-BT₁₀-	AGCGTCGGCAGTTATACATATAGCTTCGGAACAC −5'
	D17b		TATATCGAAGCCTTGTG −3'
1nt gap	17e	5'-	TCGCAGCCGYCAATATG
	T1b	3'-BT₁₀-	AGCGTCGGCAGTTATACATATAGCTTCGGAACAC −5'
	D16b		ATATCGAAGCCTTGTG −3'
2nt gap	17e	5'-	TCGCAGCCGYCAATATG
	T1b	3'-BT₁₀-	AGCGTCGGCAGTTATACATATAGCTTCGGAACAC −5'
	D15b		TATCGAAGCCTTGTG −3'
3nt gap	17e	5'-	TCGCAGCCGYCAATATG
	T1b	3'-BT₁₀-	AGCGTCGGCAGTTATACATATAGCTTCGGAACAC −5'
	D14b		ATCGAAGCCTTGTG −3'
4nt gap	17e	5'-	TCGCAGCCGYCAATATG
	T1b	3'-BT₁₀-	AGCGTCGGCAGTTATACATATAGCTTCGGAACAC −5'
	D13b		TCGAAGCCTTGTG −3'
Primer/template	17e	5'-	TCGCAGCCGYCAATATG
	T1b	3'-BT₁₀-	AGCGTCGGCAGTTATACATATAGCTTCGGAACAC −5'
Primer/template-	17e	5'-	TCGCAGCCGYCAATATg
mismatch	T2b	3'-BT₁₀-	AGCGTCGGCAGTTATAGATATAGCTTCGGAACAC −5'