Appendix 4—table 1. Steady-state fluorescence controls.

Labeled macromolecule	Label	Ligand	Normalized intensity ${}^{a,b}$	Anisotropy ${}^a$
Double flap	A488	none	1.0	0.05 ± 0.01
		Pol I	0.94	0.21 ± 0.05
Primer/template	A488	none	1.0	0.05 ± 0.01
		Pol I	1.2	0.22 ± 0.01
Pol I	A594	none	1.0	0.32 ± 0.02
		double flap	1.2	0.34 ± 0.03
		primer/template	1.1	0.34 ± 0.03

${}^a$ total emission intensity normalized to the value for the free macromolecule ${}^b$ A488 data obtained with excitation at 495 nm, A594 with excitation at 590 nm.