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. 2021 Feb 26;47(5):67. doi: 10.3892/ijmm.2021.4900

Figure 3.

Figure 3

RA inhibits glioma cell growth. (A and C) U251 and U343 cells were treated with the indicated doses of RA for 24 and 48 h, viability was determined using the CCK-8 assay, and data in control cells were normalized to 100%. Data are presented as the means ± SD of three experiments. (B and D) The morphological changes of U251 and U343 cells. U251 and U343 cells were treated with various doses of RA for 24 and 48 h and observed using inverted phase-contrast microscopy. Scale bar, 100 µm. (E) The viability of NHA was determined after treatment with the indicated doses of RA for 24 and 48 h. (F) Cell morphology of NHA after treatment with the indicated doses of RA for 24 and 48 h. Scale bar, 100 µm. *P<0.05, **P<0.01 and ***P<0.001 vs. the DMSO group. RA, rosmarinic acid.