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. 2021 Feb 26;47(5):67. doi: 10.3892/ijmm.2021.4900

Figure 7.

Figure 7

The PI3K/Akt/NF-κB signaling pathway is involved in RA-induced cytotoxicity in glioma cells. (A) The protein expression of PI3K, p-Akt, Akt, and NF-κB p65 in U251 was assessed by western blotting. (B) Densitometric quantification of the bands in A. (C) The protein expression of PI3K, p-Akt, Akt, and NF-κB p65 in U343 was assessed by western blotting. (D) Densitometric quantification of the bands in C. (E and H) Expression levels of NF-κB p65 in the nucleus were detected by western blotting. (F and I) Densitometric quantification of the bands in E and H. (G and J) Viability of U251 and U343 cells as measured by CCK-8 assay. The results are expressed as the mean ± SD. GAPDH was used as a loading control. *P<0.05, **P<0.01 and ***P<0.001 vs. the DMSO group; #P<0.05 and ##P<0.01 vs. the RA treatment group. RA, rosmarinic acid.