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. 2021 Mar 1;47(5):70. doi: 10.3892/ijmm.2021.4903

Figure 6.

Figure 6

Mature miR-503 induces keloid-derived fibroblast proliferation and accumulation of extracellular matrix but inhibits apoptosis. (A) Knockdown efficiency of the miR-503-3p and miR-503-5p specific inhibitors was investigated in keloid-derived fibroblasts via reverse transcription-quantitative PCR. Viability of keloid-derived fibroblasts transfected with (B) miR-503-3p or (C) miR-503-5p inhibitor was determined by Cell Counting Kit-8 assay. (D and E) Proliferation of miR-503-3p or miR-503-5p inhibitor-transfected keloid-derived fibroblasts was measured by colony formation assay. (F and G) Apoptosis and (H) cell cycle progression in keloid-derived fibroblasts transfected with (I) miR-503-3p or (J) miR-503-5p inhibitor was assessed by flow cytometry. Western blot assays were used to determine the expression levels of extracellular matrix-associated proteins in keloid-derived fibroblasts transfected with (K and L) miR-503-3p or (M and N) miR-503-5p inhibitor. **P<0.01 vs. Ctrl. miR, microRNA; Ctrl, control; OD, optical density; SMA, smooth muscle actin.