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. 2021 Feb 26;12:618023. doi: 10.3389/fphar.2021.618023

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Copyright © 2021 Gao, Ma, Luo, Li, Jiang, Jiang, Pi, Chen, Chen, Zhang, Zheng and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemistry Results of ACE 6–8 weeks C57/B6 mice, with or without Nrf2 knockout were exposed to either filtered air or air containing particulate matter (PM) for six weeks, and then the vascular tissues were collected, embedded in paraffin and subjected to immunohistochemistry for ACE. N = 6 per group. WTC was C57 wild-type control group, WTE was C57 wild-type PM exposure group, KOC was Nrf2-/- group control group, and KOE was Nrf2-/- group PM exposure group. Scale bars are 1,000 μm and magnification is 400×. *: statistically different between the two groups (p < 0.05) (A): Immunohistochemistry Results of ACE in WTC group (B): Immunohistochemistry Results of ACE in WTE group (C): Immunohistochemistry Results of ACE in KOC group (D): Immunohistochemistry Results of ACE in KOE group (E): Quantification of the ACE expression.