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. 2021 Feb 26;12:577766. doi: 10.3389/fimmu.2021.577766

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Copyright © 2021 Ocadlikova, Lecciso, Broto, Scotlandi, Cavo, Curti and Palmerini

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometry analysis of DC-maturation. SaOS-2 and SYO-1 cell lines were treated (SaOS-2+SUN and SYO-1+SUN) or left untreated (SaOS-2 and SYO-1) for 24 h with sunitinib (SUN) at concentrations of 20 μM for SaOS-2 and 15 μM for SYO-1 cell lines, and used for monocyte-derived DC pulsing for another 24 h at a ratio of 1:2 (DC:sarcoma cell). DCs pulsed with untreated sarcoma cells were irradiated. The immunophenotype was analyzed by flow cytometry. Unloaded DCs (w/o SaOS-2/SYO-1) were used as negative control. The values are represented as mean ± SEM of 5 independent experiments. **p < 0.01; ***p < 0.001; ****p < 0.0001 compared to unloaded DCs.