Figure 4.

Flow cytometry analysis of Tregs induced by DCs loaded with sunitinib treated sarcoma cells. SaOS-2 and SYO-1 cell lines were left untreated or treated for 24 h with sunitinib (SUN) at concentrations of 20 μM and 15 μM, respectively, and used for monocyte-derived DC pulsing for another 24 h at a ratio of 1:2 (DC:sarcoma cell). DCs pulsed with untreated sarcoma cells were irradiated. DCs loaded with untreated SaOS-2/SYO-1 or sunitinib-treated cells were then mixed with autologous CD3⁺ T cells at a ratio of 1:10 (DC:T cell). Unstimulated CD3⁺ T cells were used as negative control. After 5 days, the percentage of CD3⁺CD4⁺CD25^highCD127^low/-FOXP3⁺ Tregs was evaluated by flow cytometry. The values are represented as mean ± SEM of 5 independent experiments. *p < 0.05; **p < 0.01.