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. 2021 Feb 26;12:577766. doi: 10.3389/fimmu.2021.577766

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Copyright © 2021 Ocadlikova, Lecciso, Broto, Scotlandi, Cavo, Curti and Palmerini

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometry analysis of PD-1 expression on T cells induced by DCs loaded with sunitinib treated sarcoma cells. SaOS-2 and SYO-1 cell lines were treated for 24 h with sunitinib (SUN) at concentrations of 20 μM and 15 μM, respectively, and used for monocyte-derived DC pulsing for another 24 h at a ratio of 1:2 (DC:sarcoma cell). Then, DCs loaded with untreated and irradiated SaOS-2/SYO-1, or sunitinib-treated cells (DCs+SaOS-2/SYO-1+SUN) were mixed with autologous CD3⁺ T cells at a ratio of 1:10 (DC:T cell) After 5 days, the percentage of PD-1 on (A) CD4⁺ and CD8⁺ T cells, (B) naïve (N), central memory (CM), effector memory (EM), and EM expressing RA (EMRA) CD4⁺ and CD8⁺T cells and (C) Tregs was evaluated by flow cytometry. The values are represented as mean ± SEM of 5 independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001.