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. 2021 Feb 26;12:616409. doi: 10.3389/fphar.2021.616409

FIGURE 5.

FIGURE 5

GRb1 protects zebrafish larvae against oxidative stress induced by alcohol exposure. (A) Fluorescence micrographs of DHE. Figures are magnified as 100ⅹ. (B) ROS quantification according to the mean intensity of red fluorescence using ImageJ software (n = 6–8 per group). (C) Fluorescence micrographs of NA-8. Figures are magnified as 100ⅹ. (D) GSH quantification according to the mean intensity of blue fluorescence using ImageJ software (n = 6–8 per group). Data are presented as the mean ± SEM. p < 0.05 (#), p < 0.01 (##), and p < 0.001 (###) compared the control group; p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***) compared with the 350 mM EtOH group.