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. 2021 Mar 11;11:5763. doi: 10.1038/s41598-021-85050-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Size of cells attached to bioactive glass monoliths with different nanopore size and HA/CHA microstructure. The actin cytoskeleton of MC3T3-E1 cells attached after 2 h to monoliths was stained with Alexa488-conjugated phalloidin and imaged by fluorescence microscopy. (a) Representative black and white images of the actin cytoskeleton of cells attached on long-needle (LN), plate-like (PL), flower-like (FW), and short-needle (SN) HA/CHA-covered monoliths (scale bar = 100 µm). (b) Quantitative analyses of the average size of attached cells on the HA/CHA layer determined by measuring the stained area covered by cells in multiple images of three independent experiments. Significant differences in cell numbers are marked with *.