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. 2021 Mar 11;11:5775. doi: 10.1038/s41598-021-85166-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of TCs using TEM. Ultrathin sections of the neck skin of day 5 quail embryos. (A) TCs (blue colored) identified by TPs and podoms (red circles). TCs formed an extensive 3D network. Note the sprouting endothelial cells (brown colored) that occurred after constriction (asterisk). TCs established contact with the endothelial cells (arrowhead). (B) TCs (blue colored) established contact with the endothelial cells (arrowhead). Note the secretory vesicles (v).