Table 3.

Identity, sources, and working dilution of antibodies used in immunohistochemical studies.

Target	Primary antibody supplier	Origin (catalog no)	Dilution	incubation	Antigen retrieval	secondary antibody-incubation time
CD34	MOUSE ANTI CHICKEN CD34 (Bio rad)	MOUSE ANTI CHICKEN CD34 Monoclonal Antibody (Clone: AV138) (Cat.no MBS224490)	1:100	Over night	boiling in citrate buffer (pH 6.0), 20 min Goat	Goat anti-Mouse IgG (H + L) Secondary Antibody Catalog # 31,569 Dilution ; 1:100 One hour at room temperature
VEGF	Rabbit anti -VEGF (Invitrogen by Thermo Fisher Scientific Waltham, MA, USA))	Rabbit VEGF Polyclonal Antibody (clone: RB-222-P0) (Cat.no PA1-21,796)	1:100	Overnight	boiling in citrate buffer (pH 6.0), 20 min	Goat anti-rabbit secondary antibody (cat. no. K4003, EN Vision System Horseradish Peroxidase Labelled Polymer; Dako) Ready to use 30 min at room temperature
CD68 (Macrophage Marker) Ab-3 (Clone KP1)	Mouse Anti-CD68 Thermo Fisher Scientific Lab Vision Corporation, Fremont, USA	Mouse Monoclonal Antibody Cat. #MS-397-R7	1:100	Overnight	boiling in citrate buffer (pH 6.0), 20 min
MMP9 / Gelatinase B	Rabbit anti-MMP9 LifeSpan BioSciences	Rabbit Polyclonal antibody Catalog # LS-C31757	1:100	Overnight	boiling in citrate buffer (pH 6.0), 20 min

Antibodies used that showed reactivity in Avian species.