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. 2021 Mar 11;11:5690. doi: 10.1038/s41598-021-85055-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The morphology of the 2XSB sporadic MPNST cell line recapitulates the pattern seen in the parent tumor. (a–d) Representative brightfield (a) and phase contrast (b–d) images of logarithmic phase 2XSB cells. Both brightfield and phase contrast microscopy show that the tumor cells have a cobblestoned to spindled morphology. Note also that the ×60 phase contrast images in (c) show several cytoplasmic vacuoles in the tumor cells, while (d) illustrates two representatives of the subpopulation of large multinucleated cells present in this line (arrows). (e–j) Two representative 3D culture images (g,j) show the merged nuclei and cytoskeleton images acquired at ×63 with z-stack microscopy highlighting spindled (e–g) and polygonal (h–j) multinucleated cells. Panels (e–i) show the individual channels acquired for phallodin-Texas Red (cytoskeleton labeling, f,i) and DNA-bisbenzamide (nucleus labeling, e,h) prior to merging in panels (g) and (j). (k–m) Transmission electron microscopy (k) demonstrates that 2XSB cells contain swollen (1) and normal (2) mitochondria and swollen rough endoplasmic reticulum (3). Both 2XSB cells and non-neoplastic human Schwann cells also have numerous microvilli on their cellular surfaces (1 in l,m).