Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 11;11:5690. doi: 10.1038/s41598-021-85055-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Growth characteristics of 2XSB MPNST cells in 2D cultures, 3D cultures, clonogenic assays and xenografts. (a) In vitro doubling time of both low (P6) and high passage (P54) cultures shows a consistent rate of proliferation in these passages. (b) Representative image of a 3D culture of 2XSB cells grown for 1-week post-plating on Matrigel demonstrating the high degree of invasive projections into Matrigel characteristic of this line (arrow). (c) Representative image of a clonogenic round bottom spheroid assay 1-week post-plating showing that 2XSB cells have the ability to survive and proliferate when embedded in Matrigel, resulting in the formation of colonies. These colonies also showed multiple regions of tumor cells invading the Matrigel (arrows). (d) A ×40 image of a hematoxylin and eosin stained section of a xenograft derived from 2XSB cells showing that the xenograft has histologic features resembling those of the parent tumor. Arrows indicate some of the numerous mitotic figures present in this xenograft.