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. 2021 Feb 26;9:623753. doi: 10.3389/fcell.2021.623753

FIGURE 3.

FIGURE 3

Ablation of WNT β-catenin pathway does not alter primary ciliogenesis. (A) Western blot analysis of individual HEK293 KO cell lines using the indicated antibodies. Note that LRP5/6 dKO cells lack LRP6 and phospho LRP5/6 (pSer1493/pSer1490), DVL1/2/3 tKO cell do not have detectable levels of DVL2. AXIN1/2 dKO cells shown elevated level of ABC. (B–D) HEK293 Cells were starved for 48 h, stained for Arl13b (green), γ-tubulin (red), and DAPI (blue), and analyzed by IF microscopy. Representative images are shown in (B). Scale bar = 2 μm. Quantification of cilia length and percentage of ciliated cells is shown in (C,D), respectively Each dot indicates either length of a single primary cilium (C) or percentage of ciliated cells in one image (D). n = 4.