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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: J Biomed Mater Res A. 2020 Sep 21;109(7):1080–1087. doi: 10.1002/jbm.a.37100

Figure III: Osteogenic Potential of CDHAp.

Figure III:

Ct: control group, HAp: 2ml/ml CDHAp and SB: 2mg/ml commercially available HA (Sigma).) * indicated a statistical difference between groups. All experiments were performed using biological triplicates and technical triplicates (n=9) (a) After 14 days of culture in osteogenic media, a significantly lower (p<0.05) DNA concentration in the samples containing CDHAp compared to the samples containing SBp or no HA (b) Metabolic activity of cells after 0 and 14 days of culture. The results are normalized to DNA content. Results showed a significant increase (p<0.05) in metabolic activity at D14 in the CDHAp group. (c) mRNA fold change for BMP2 and OCN after 7 and 14 days of culture. Rt-PCR showed an increase in gene expression of BMP2 and OCN in MSCs cultured with both HAp (CDHAp and SBp) after 14 days (d) Von Kossa histology staining shows great calcification (black) in SBHAp cells on Day 14 compared to SBp. Cells are stained pink.