. Author manuscript; available in PMC: 2022 Mar 12.

Published before final editing as: J Microbiol Methods. 2020 Sep 12;178:106058. doi: 10.1016/j.mimet.2020.106058

Table 3.

Nucleotides used in this study

Name	Sequence

4767
cctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacg ttgtaaaacgacggccagtgccaagcttgcatgcctgcaggtcgactctagagctacattcgggtgaactataatctgaacggcgcattaatcgacgcc gtgctgatgctgatcgaacagcaaatggccgcgctggaacaggaagaaagcccgctttcattgagctcagaagatatccaactctatgaaaaacaattgaa atcaagtgattactatgggctgtttgtcgatacagtacccgacgatgtcaaaaaactgtatactgaggcgggcagcagtgatttcaatgcgctgtcacaaacc gcacaccgcctgaaaggcgtgtttgccatgttaaatctgcttcccggcaagcagctgtgcgaatcgttagaacagcgcatcgcagaaggtgatgcgcccga gatcgagaataacatcagtcagattgattttttcgtcagcagactgctgaagcaaggtagccaacaacatgaataacctgaacgtaattattgctgatgaccat cctatcgtactgtttggcatccggaagtcacttgagcaaattgaatgggaggacctaggaatggtatcaaaaggggaagaactttttaccggggtagtaccaa ttcttgttgaattggatggtgacgtgaacggccacaagttcagcgtacggggagagggcgaaggtgacgccacgaatgggaaacttacactgaaatttatct gcacaaccggaaaactccccgttccctggccaacattggttacgacattcgggtacggggttgcctgcttcagtcggtacccggaccatatgaagcaacac gatttctttaagtctgccatgccggagggttatgtccaggaacggactatttcattcaaagacgacggaacatataaaacccgtgcggaagttaagtttgagg gcgataccttggtaaatcgcattgaactcaagggcattggaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctgga caagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccg gaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttcttgccgccaaggatctgatggcgcaggggatcaa gatctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatg actgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgcc ctgaatgaactccaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagg gactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctg catacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatga tctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcggatgcccgacggcgaggatctcgtcgtgacccatggcg atgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcagga
4768
gactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctg tccggtgccctgaatgaactccaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaag cgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatg cggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcg atcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcggatgcccgacggcgaggatctcgtcgtg acccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggac atagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcg ccttctatcgccttcttgacgagttcttctgagcgggactctggggtagttacgctagggataacagggtaatataggccgcgaagttcctattccgaagttcc gaaatttatctgcacaaccggaaaactccccgttccctggccaacattggttacgacattcgggtacggggttgcctgcttcagtcggtacccggaccatatg aagcaacacgatttctttaagtctgccatgccggagggttatgtccaggaacggactatttcattcaaagacgacggaacatataaaacccgtgcggaagtta agtttgagggcgataccttggtaaatcgcattgaactcaagggcattgatttcaaagaggatgggaatatcctgggtcacaagttggaatataattttaattcac actacgtgtacattaccgctgataaacagaaaaactgcataaaggctaacttcaaaatacgccataatgtggaggacgggtcagtacaactcgcagaccatt accaacagaacacaccaataggtgacggaccagtgcttctgcccgataaccattatctgtcccatcaaagcaagctctcgaaagatccgaacgagaaacg ggatcacatggtattgttggagtttgtcactgccgctggaattacacacggaatggatgagctgtataagtaaggatccagcccgcctcacggcaggttaac gccaaacaggcacggcatccgccgtgcctgtttgcttttcagacctcgctgcggttgtagcgtacctgctggctgtaataagccagcgtctgctccagcgtct ccagcgtaaccggcttcgacaggcaattatccatgcccgcctcgaggcagcgctgtttctcttccgccaacgcgttcgccgtcacgccgatcaccggcgcg ctgaactgcatctgccgcagttcctgcgtcagacgatagccgtccatgttcggcatattgacgtcggtcagcacaatatcgacgtggtgctgtttcagcacgc cgagcgcgtcgacgccgtcgttggcggtcaccacctgataccccagcgaccccagctgatcggacaacaggcgacggttgatcggatgatcgtcaacca ccagcagatgaatatcgccgttgtccgccgcactggccttggcgggcaccggcagctgtaccagcgcctctggggtaccgagctcgaattcgtaatcat ggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtg
4876	tcgacggtatcgataagcttgatatcgaattcctgcagatggtatcaaaaggggaagaac
4877	caccgcggtggcggccgctctagaactagtggatcccttacttatacagctcatccattc

All nucleotides are listed as 5’ to 3’. Bolded sequence directs recombination with vector pMQ132. Underlined sequences direct recombination between dsDNA sequences.