Figure 2.

MCPIP-1 is rapidly induced and preceded spontaneous neutrophil apoptosis. (A–C) Neutrophils were isolated from human blood and cultivated as indicated. (A) MCPIP-1 transcript level was determined using qPCR. Bars represent the mean relative expression to time 0 (t₀ = 1) ± SD; each symbol indicates different donor (B) Pearson's correlation coefficient values were calculated based on MCPIP-1 and IL-8 transcript levels. n = 6 (C) MCPIP-1 transcript level was determined using qPCR (circle). Cells were double-stained with Annexin V and PI (triangle), and analyzed using flow cytometry. A representative result was shown ± SEM. PI, propidium iodide. p-value of < 0.01 (**); p-value of < 0.001 (***).