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. 2021 Feb 26;12:627922. doi: 10.3389/fimmu.2021.627922

Table 1.

List of oligonucleotides used in the studies.

Oligonucleotide Sequence Temperature program
1 —denaturation,
2 —annealing,
3 —extension
m_Ef2_R 5′-TCAGCACACTGGCATAGAGGC-3′ 1.95°C, 20 s2. 6
2°C, 20 s
3. 72°C, 30 s
m_Ef2_F 5′-GACATCACCAAGGGTGTGCAG-3′
m_Mcpip-1_R 5′-CAGCCGCTCCTCGATGAAGC-3′
m_Mcpip-1_F 5′-CAGCCTCGACCAGATGTGCC-3′
m_Bid_R 5′-CACTCAAGCTGAACGCAGAG-3′ 1.95°C, 30 s
2. 57°C, 30 s
3. 72°C, 30 s
m_Bid_F 5′-TGTGAGGAACTTGGTTAGAAACG-3′
m_Bcl2a1_R 5′-TGCTGCATTGTTCCCGTAGA-3′
m_Bcl2a1_F 5′-GCATCGTGGCCTTTTTCTCC-3′
m_Mcl-1_R 5′-TAAGGACGAAACGGGACTGG-3′
m_Mcl-1_F 5′-TAAGGACGAAACGGGACTGG-3′
m_Bim_R 5′-CCTGTGCAATCCGTATCTCCG-3′ 1.95°C, 30 s
2. 64°C, 30 s
3. 72°C, 30 s
m_Bim_F 5′-GCCAGGCCTTCAACCACTATC-3′
EF2_R 5′-TTCAGCACACTGGCATAGAGGC-3′ 1.95°C, 30 s
2. 62°C, 30 s
3. 72°C, 30 s
EF2_F 5′-GACATCACCAAGGGTGTGCAG-3′
MCPIP-1_R 5′-TCCAGGCTGCACTGCTCACTC-3′
MCPIP-1_F 5′-GGAAGCAGCCGTGTCCCTATG-3′
IL-8_R 5′-TCTCAGCCCTCTTCAAAAACTTCT-3′ 1.95°C, 20 s
2. 60°C, 1 min
3. 72°C, 45 s
IL-8_F 5′-ATGACTTCCAAGCTGGCCGTGGCT-3′
MCL-1_R 5′-CCAGCTCCTACTCCAGCAA-3′ 1.95°C, 20 s
2. 58°C, 1 min
3. 72°C, 1 min
MCL-1_F 5′-TAAGGACAAAACGGGACTGG-3′
BAK-1_R 5′-GTCAGGCCATGCTGGTAGAC-3′ 1.95°C, 15 s
2. 58°C, 15 s
3. 72°C, 20 s
BAK-1_F 5′-CATCAACCGACGCTATGACTC-3′
BID_R 5′-CTTTGGAGGAAGCCAAACAC-3′
BID_F 5′-CCATGGACTGTGAGGTCAAC-3′
BAX_R 5′-TCAGCCCATCTTCTTCCAGA-3′ 1.95°C, 15 s
2. 60°C, 30 s
3. 72°C, 30 s
BAX_F 5′-GCTGTTGGGCTGGATCCAAG-3′
BCL2A1_R 5′-ACAAAGCCATTTTCCCAGCCT-3′
BCL2A1_F 5′-AAATTGCCCCGGATGTGGAT-3′
BCL2L1_R 5′-ACAAAAGTATCCCAGCCGCC-3′
BCL2L1_F 5′-CTGTGCGTGGAAAGCGTAGA-3′
BCL3_R 5′-ACATTTGCGCGTTCACGTT-3′
BCL3_F 5′-TCGACGCAGTGGACATTAAGAG-3′
RELB_R 5′-GAACATGTTGCTGCCCACAAG-3′
RELB_F 5′-CATCCTGGACCACTTCCTGCC-3′