Table 1.
Oligonucleotide | Sequence | Temperature program |
---|---|---|
1 —denaturation, 2 —annealing, 3 —extension |
||
m_Ef2_R | 5′-TCAGCACACTGGCATAGAGGC-3′ | 1.95°C, 20 s2. 6 2°C, 20 s 3. 72°C, 30 s |
m_Ef2_F | 5′-GACATCACCAAGGGTGTGCAG-3′ | |
m_Mcpip-1_R | 5′-CAGCCGCTCCTCGATGAAGC-3′ | |
m_Mcpip-1_F | 5′-CAGCCTCGACCAGATGTGCC-3′ | |
m_Bid_R | 5′-CACTCAAGCTGAACGCAGAG-3′ | 1.95°C, 30 s 2. 57°C, 30 s 3. 72°C, 30 s |
m_Bid_F | 5′-TGTGAGGAACTTGGTTAGAAACG-3′ | |
m_Bcl2a1_R | 5′-TGCTGCATTGTTCCCGTAGA-3′ | |
m_Bcl2a1_F | 5′-GCATCGTGGCCTTTTTCTCC-3′ | |
m_Mcl-1_R | 5′-TAAGGACGAAACGGGACTGG-3′ | |
m_Mcl-1_F | 5′-TAAGGACGAAACGGGACTGG-3′ | |
m_Bim_R | 5′-CCTGTGCAATCCGTATCTCCG-3′ | 1.95°C, 30 s 2. 64°C, 30 s 3. 72°C, 30 s |
m_Bim_F | 5′-GCCAGGCCTTCAACCACTATC-3′ | |
EF2_R | 5′-TTCAGCACACTGGCATAGAGGC-3′ | 1.95°C, 30 s 2. 62°C, 30 s 3. 72°C, 30 s |
EF2_F | 5′-GACATCACCAAGGGTGTGCAG-3′ | |
MCPIP-1_R | 5′-TCCAGGCTGCACTGCTCACTC-3′ | |
MCPIP-1_F | 5′-GGAAGCAGCCGTGTCCCTATG-3′ | |
IL-8_R | 5′-TCTCAGCCCTCTTCAAAAACTTCT-3′ | 1.95°C, 20 s 2. 60°C, 1 min 3. 72°C, 45 s |
IL-8_F | 5′-ATGACTTCCAAGCTGGCCGTGGCT-3′ | |
MCL-1_R | 5′-CCAGCTCCTACTCCAGCAA-3′ | 1.95°C, 20 s 2. 58°C, 1 min 3. 72°C, 1 min |
MCL-1_F | 5′-TAAGGACAAAACGGGACTGG-3′ | |
BAK-1_R | 5′-GTCAGGCCATGCTGGTAGAC-3′ | 1.95°C, 15 s 2. 58°C, 15 s 3. 72°C, 20 s |
BAK-1_F | 5′-CATCAACCGACGCTATGACTC-3′ | |
BID_R | 5′-CTTTGGAGGAAGCCAAACAC-3′ | |
BID_F | 5′-CCATGGACTGTGAGGTCAAC-3′ | |
BAX_R | 5′-TCAGCCCATCTTCTTCCAGA-3′ | 1.95°C, 15 s 2. 60°C, 30 s 3. 72°C, 30 s |
BAX_F | 5′-GCTGTTGGGCTGGATCCAAG-3′ | |
BCL2A1_R | 5′-ACAAAGCCATTTTCCCAGCCT-3′ | |
BCL2A1_F | 5′-AAATTGCCCCGGATGTGGAT-3′ | |
BCL2L1_R | 5′-ACAAAAGTATCCCAGCCGCC-3′ | |
BCL2L1_F | 5′-CTGTGCGTGGAAAGCGTAGA-3′ | |
BCL3_R | 5′-ACATTTGCGCGTTCACGTT-3′ | |
BCL3_F | 5′-TCGACGCAGTGGACATTAAGAG-3′ | |
RELB_R | 5′-GAACATGTTGCTGCCCACAAG-3′ | |
RELB_F | 5′-CATCCTGGACCACTTCCTGCC-3′ |