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. 2021 Feb 26;12:557514. doi: 10.3389/fphys.2021.557514

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Copyright © 2021 Hagdorn, Kurakula, Koop, Bossers, Mavrogiannis, van Leusden, van der Feen, de Boer, Goumans and Berger.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Long-term volume loading does not induce myocardial fibrosis. (A,B) mRNA levels of Col1a for both ventricles. (C) Protein levels of RV collagen 1. (D,E) Results of fibrosis quantification on Masson’s trichrome stainings, expressed as percentage blue-stained area. (F) Representative examples of Masson’s trichrome fibrosis stainings. White bars represent 200 μm, and black bars represent 4 mm. (G) Representative examples of Sirius red fibrosis stainings. White bars represent 200 μm, and black bars represent 4 mm. * represents significantly different from control, and bar represents significantly different between ACS groups.