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. 2021 Feb 26;9:644397. doi: 10.3389/fcell.2021.644397

FIGURE 4.

FIGURE 4

miR-206 inhibitor rescued the function of circ_0057558 knockdown, while miR-206 mimics rescued the function of circ_0057558 overexpression. (A–C) 22RV1 cells were transduced with lentivirus expressing circ_0057558 shRNA (shcirc#1)/control shRNA (shNC#1) and transfected with miR-206 inhibitor (miR-inh)/control (miR-NC). miR-206 expression (A) was detected by qRT-PCR at 48 h post treatment. CCK-8 assay (B) and cell cycle analysis (C) was carried out to detect cell proliferation and cell cycle distribution, respectively. *P < 0.05, ***P < 0.001 versus shNC#1 + miR-NC; #P < 0.05, ##P < 0.01, ###P < 0.001 versus shcirc#1 + miR-NC. (D–F) PC3 cells were transfected with plasmid expressing circ_0057558 (circOE)/control vector (control) and transfected with miR-206 mimics (miR-mimics)/control (miR-NC). qRT-PCR (D), CCK-8 assay (E), and cell cycle analysis (F) were done to detect miR-206 expression, cell proliferation, and cell cycle distribution, respectively. $P < 0.05, $$$P < 0.001 versus control + miR-NC; &&&P < 0.001 versus circOE + miR-NC.