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. 2021 Feb 26;9:644397. doi: 10.3389/fcell.2021.644397

FIGURE 5.

FIGURE 5

circ_0057558 regulated the miR-206 target, USP33. (A) mRNA expression of USP33 in 22RV cells infected with lentivirus expressing circ_0057558 shRNA (shcirc#1) or transfected with miR-206 mimics (miR-mimics). Cells infected with control shRNA (shNC#1) and transfected with control miRNA (miR-NC) were served as control. ###P < 0.001 versus shNC#1 + miR-NC. (B) The putative miRNA binding sites in the USP33 3′UTR. (C) pGL3-USP33 wild-type (WT) or mutant was co-transfected into 22RV1 cells with miR-206 mimics (miR-mimics) or control (miR-NC). Luciferase assay was performed to determine luciferase activity. The relative luciferase activity normalized to the control group (miR-NC). ***P < 0.001 versus miR-NC. (D) qRT-PCR analysis following the RIP assay was conducted to confirm the interaction between miR-206 with USP33. $$$P < 0.001 versus IgG. (E) Expression of USP33 mRNA in prostate cancer tissue and adjacent non-tumor tissue. (F,G) Pearson r analysis showed the correlation between the expression of miR-206 and USP33 and the expression of circ_0057558 and USP33 in prostate cancer tissues.