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. 2021 Feb 26;9:644397. doi: 10.3389/fcell.2021.644397

FIGURE 6.

FIGURE 6

USP33 overexpression rescued the function of miR-206 mimics. (A) Protein expression of USP33 in PC3 cells infected with lentivirus expressing USP33 (USP33OE)/control vector (Vector). Representative blots from three independent experiments are shown. (B–D) PC3 cells were transfected with USP33OE/vector and transfected with miR-206 mimics (miR-mimics)/control (miR-NC). CCK-8 assay (B) and cell cycle analysis (C) were done to detect cell proliferation and cell cycle distribution, respectively. (D) CyclinD1 and CyclinB1 were detected by western blotting. **P < 0.01, ***P < 0.001 versus miR-NC + vector; #P < 0.05, ###P < 0.001 versus miR-mimics + vector.