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. 2021 Feb 26;9:644397. doi: 10.3389/fcell.2021.644397

FIGURE 7.

FIGURE 7

USP33 deubiquitinated c-Myc. (A–C) 22RV1 cells were transfected with USP33 siRNAs (si#1 and si#2) or control siRNA (siNC). After 48 h, the protein expression of USP33 (A) and c-Myc (B) and the mRNA expression of c-Myc (C) were evaluated. (D) 22RV1 cell lysate was immunoprecipitated (IP) with anti-USP33 (upper) or anti-c-Myc (lower) or control IgG and then immunoblotted (IB) with anti-USP33 and anti-c-Myc. (E) 22RV1 cells were transfected with USP33 siRNA (si#1) or siNC. Immunoprecipitation experiment was performed with anti-c-Myc or control IgG and IB with anti-ubiquitin (Ub). (F) 22RV1 cells were transduced with lentivirus expressing circ_0057558 shRNA (shcirc#1)/control shRNA (shNC#1) and transfected with miR-206 inhibitor (miR-inh)/control (miR-NC). The expression of USP33 and c-Myc was detected at 48 h post treatment. (G) PC3 cells were transfected with plasmid expressing circ_0057558 (circOE)/control vector (control) and miR-206 mimics (miR-mimics)/control (miR-NC). The expression of USP33 and c-Myc was assessed at 48 h post treatment. (H) 22RV1 cells were transduced with shcirc#1/shNC#1 and USP33OE/vector. The expression of USP33 and c-Myc was detected at 48 h post treatment.