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. 2021 Feb 26;9:644397. doi: 10.3389/fcell.2021.644397

FIGURE 8.

FIGURE 8

circ_0057558 expression level was correlated with the proliferation inhibition effect of c-Myc inhibitor MYCi361. (A–C) Primary prostate cancer cells were isolated as described in Materials and Methods section. circ_0057558 expression (A) in the primary prostate cancer cells was assessed by qRT-PCR. Primary cells were treated with 10 nM docetaxel (B), 6 μM MYCi361 (C), or vehicle (DMSO) for 48 h. Inhibition rate of cell proliferation (%) was determined using CCK-8 assay. (D–G) Patient-derived xenograft (PDX) model was established with fresh prostate cancer tissues and defined as circ_0057558high group and circ_0057558low group. The F2 PDXs were treated with MYCi361 (55 mg/kg/day) and vehicle (DMSO) twice a week by intraperitoneal injection. Tumor growth curves (D) and the photos and weight of PDX (E) are shown. (F) Immunofluoresence staining with anti-Ki-67 was carried out to assess cell proliferation in xenografts. Scale bar: 50 μm. **P < 0.01, ***P < 0.001.