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. 2021 Mar 11;11:5709. doi: 10.1038/s41598-021-85319-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Morphology of Case 3. (1) Biopsy. (2) Resection specimen. (3) Sarcoma. Typical GCTB with osteoclastic giant cells and intermingled mononuclear cells in the biopsy (1A) and the resection specimen (2A) as well as neoplastic mononuclear cells with positive H3F3A G34W staining in the nuclei (1B,2B) (bar = 100 µm). Staining for Ki-67 shows about 1% of positive cells (1C,2C). Sanger-Sequencing for H3F3A G34W shows the mutation specific thymine in tissue of the biopsy (1D) as well as the resection specimen (2D). The sarcoma showed spindle to pleomorphic tumor cells and extensive necrosis (3A) and a high Ki-67 index (3C), however it did not stain positive for H3F3A G34W (3B). Sanger-sequencing of DNA from microdissected tissue of the H3F3A G34W-negative tumor showed a very weak peak for a mutation-specific thymine (3D).