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. 2021 Mar 11;11:5790. doi: 10.1038/s41598-021-84171-3

Figure 1.

Figure 1

(A) Dot blot of heat-treated sEGFR detected by antibodies 528 and cetuximab. Each blotting image was a consecutive membrane strip without cropping. (B,C) Competitive Biacore assay between 528 and EGF (B) and between 528 and cetuximab (C) for binding sEGFR. sEGFR (100 nM) was loaded onto the antibody-immobilized sensor chips with or without its competitor.