Fig. 1. The DAb-seq workflow.

a Bone marrow aspirates of patients with AML contain healthy and malignant cells that exhibit diverse genotypes and immunophenotypes. These cells are stained with antibodies labeled with DNA tags. b Stained cells are paired and encapsulated with a barcode bead on a Mission Bio Tapestri instrument. c In each droplet, a PCR labels antibody tags and genomic DNA targets simultaneously with a unique cell index. d Sequencing the barcoded amplicons and antibody tags yields coupled single-cell immunophenotype and genotype data for thousands of cells.