Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 11;12:1583. doi: 10.1038/s41467-021-21810-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Three timepoints sampled with DAb-seq during treatment comprise a mixture of independent clones (KRAS G13D heterozygous blasts, yellow; FLT3 D835Y blasts, red). The wild-type compartment contains additional cells with a blast-like immunophenotype lacking detectable mutations. b Heatmap of log-transformed corrected antibody counts and genotyping calls for the KRAS and FLT3 loci for each cell across all timepoints. The heatmap is grouped by genotype. Cells with wild-type genotype but blast-like immunophenotype are labeled separately. c UMAP embedding of all cells from all timepoints based on log-transformed corrected antibody counts. Color indicates mutation status as in a. The blast compartment is overlaid with a spline approximating the gradient of the second principal component of the antibody count matrix (shown in inlet figure) and indicates a gradual change in immunophenotype. d Moving average expression of antibodies and fraction of mutated cells sorted by the second principal component of the antibody count matrix. The overlapping phenotypic continuum between the genetically distinct blast clones is apparent. Source data are provided as a Source data file.