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. 2021 Feb 26;12:632398. doi: 10.3389/fphys.2021.632398

FIGURE 4.

FIGURE 4

Upregulation of miR-130a partially counteracts LncRNA H19-induced cell proliferation, apoptosis, and inflammatory cytokine expression in HK-2 cells after H/R treatment. (A) The relative expressions of miR-130a after transfection with Ad-siH19 and miR-130a mimics. (B) HK-2 cell viability analysis after H/R treatment. (C,D) HK-2 cell apoptosis analysis after H/R treatment. (E,F) mRNA/protein expression levels of inflammatory cytokines in HK-2 cells after H/R treatment. H/R, hypoxia/reoxygenation treatment; Control, untreated cells; IL-1β, interleukin-1β; IL-6, interleukin-6; IL-10, interleukin-10; TNF-α, tumor necrosis factor α. All experiments were performed in biological triplicates (n = 6), and data were representative of three independent experiments. The results were expressed as mean ± standard deviation (SD). Statistical comparisons between two groups were evaluated with Student’s t-test. *P < 0.05, compared with the control groups; #P < 0.05, compared with the H/R groups; &P < 0.05, compared with the H/R+si-H19 groups.