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. 2021 Feb 26;12:632398. doi: 10.3389/fphys.2021.632398

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Copyright © 2021 Yuan, Li, Chu, Ye, Yang and Dong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Upregulation of BCL2L11 partially reverses the inhibition effect on cell apoptosis induced by downregulation of LncRNA H19 after H/R treatment. (A) The protein expression analysis of BCL2L11, Bax, Cyto-c, Bcl-2, and Caspase-3 by western blot assay. HK-2 cells were co-transfected with Ad-siH19 and pcDNA3.1-BCL2L11 after H/R treatment. (B) Mitochondrial membrane potential analyzed by JC-1 fluorescent probe in HK-2 cells after H/R treatment. H/R, hypoxia/reoxygenation treatment; Control, untreated cells; Ad-siH19 (siH19), Ad-siRNA-H19; vector, pcDNA3.1 vector; BCL2L11, Bcl-2 like protein 11; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. All experiments were performed in biological triplicates (n = 6), and data were representative of three independent experiments. The results were expressed as mean ± standard deviation (SD). Statistical comparisons between two groups were evaluated with Student’s t-test. *P < 0.05, compared with the control groups; ^#P < 0.05, compared with the H/R groups; ^&P < 0.05, compared with the H/R+si-H19 groups.