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. 2021 Feb 26;12:637595. doi: 10.3389/fpls.2021.637595

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Copyright © 2021 Liu, Yu, Ge, Tian, Hu and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Syringafactin production in PstDC3000 and its mutants and complementation stains. (A) Syringafactin halos around PstDC3000 and its derived stains. (B) Diameter of syringafactin halos around PstDC3000 and its derived strains. Bacteria were grown on KB plates (1.5% agar) for 24 h at 20°C before a mist of mineral oil was sprayed over. Halo diameters were measured to represent syringafactin production. Different letters in panel (B) indicate significant difference from one-way ANOVA followed by Fisher’s LSD test (p < 0.05). Error bars represented standard deviations. PstDC3000: Pseudomonas syringae pv. tomato DC3000; ΔrsmA2: the rsmA2 mutant; ΔrsmA3: the rsmA3 mutant; ΔrsmA23: the rsmA2/A3 double mutant; pRsmA2: pUCP18 containing the rsmA2 gene with native promoter; pRsmA3: pUCP18 containing the rsmA3 gene with native promoter.