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. 2021 Feb 26;12:611106. doi: 10.3389/fpls.2021.611106

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Copyright © 2021 Li, Deng, Tang, Su, Wang, Cheng, Yang, Qiu, Pu, Zhang, Liang, Yu, Wei and Long.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of QTsn/Fsn.cib-3D in two populations with different genetic environments. Fluorescence PCR typing results of the Kompetitive Allele-Specifc PCR (KASP) marker KASP_AX-110914105 in K1/K4 (A) and K1/YFM2 (D) populations. Orange circle represents lines carrying the allele of K1; blue box represents lines with the allele of K4 (A) and YFM2 (D), respectively. Effects of QTsn/Fsn.cib-3D on total spikelet number per spike (TSN) (B) and fertile spikelet number per spike (FSN) (C) in the K1/K4 population. Effects of QTsn/Fsn.cib-3D on total spikelet number per spike (TSN) (E) and fertile spikelet number per spike (FSN) (F) in the K1/YFM2 population; ^∗∗ and ^∗∗∗ represent significance at P < 0.01 and P < 0.001, respectively; AA represents the homozygous allele from K1; aa represents the homozygous allele from non-K1; Aa represents the heterozygous allele.