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. 2021 Mar 11;12:1547. doi: 10.1038/s41467-021-21685-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Representative images of (left) newly synthesized protein, (middle) ribosomes and (right) mRNA in (top) DMSO or (bottom) Colch-treated ARVMs. White arrow indicates ICD. b Nuclear:cytosolic and ICD:cytosolic ratios of mean fluorescence intensities of experiments shown in a. Newly synthesized protein: DMSO (N = 3, n = 52), Colch (N = 3, n = 55); ribosomes: DMSO (N = 2, n = 54), Colch (N = 2, n = 57; mRNA: DMSO (N = 3, n = 82), Colch (N = 3, n = 90). Statistical significance determined via two sample, two-tailed t-test. c Cartoon (left, top) depicting representative placement of perinuclear, cytosolic, and ICD ROIs for analysis in b and c. Mean polyA fluorescence intensity in the cytosol (left, middle), ICD (left, bottom), and nucleus (right) as a function of time. DMSO, n = 47 (1 h), n = 51 (3 h), n = 54 (6 h), n = 40 (24 h). Colch, n = 49 (1 h), n = 47 (3 h), n = 50 (6 h), n = 47 (24 h). Statistical significance determined via one-way ANOVA with post hoc Bonferroni comparison. d Representative images of (left) newly synthesized protein and (right) mRNA in (top) DMSO or (bottom) Latrunculin A-treated ARVMs. White arrow indicates ICD. e Nuclear:cytosolic and ICD:cytosolic ratios of mean fluorescence intensities of experiments shown in d. Newly synthesized protein: DMSO (N = 2, n = 46), Blebb (N = 2, n = 49), Y27632 (N = 2, n = 48), DMSO (N = 3, n = 82), LatA (N = 3, n = 80); mRNA: DMSO (N = 3, n = 70), Blebb (N = 3, n = 63), Y27632 (N = 3, n = 74), and DMSO (N = 3, n = 77), LatA (N = 3, n = 59). Statistical significance determined via one-way ANOVA with post hoc Bonferroni comparision (DMSO, Blebb, Y27632) and two sample, two-tailed t-test (DMSO, LatA). For all graphs shown in figure, the mean line is shown, with whiskers denoting standard error (SE) from the mean. Source data are provided as a Source data file.