FIGURE 3.

Circ‐ERBB2 reversed the suppressive effect of propofol on lung cancer cell progression. (a) circ‐ERBB2 expression was measured by qRT‐PCR in H1299 and A549 cells exposed to different doses of propofol (5, 10, and 15 μg/ml) for 48 h. (b) The overexpression efficiency of circ‐ERBB2 was determined by qRT‐PCR. (c–j) H1299 and A549 cells were introduced with pCD5‐ciR or circ‐ERBB2, followed by treatment with propofol (10 μg/ml) for 48 h. Circ‐ERBB2 expression (c), cell viability (d), colony number (e), apoptosis rate (f) and cell invasion (g) were detected by qRT‐PCR, CCK‐8, colony formation, flow cytometry and transwell assays. (h–j) Relative glucose consumption, lactate production and ATP/ADP ratios were examined via the appropriate kits. **p < 0.01, ***p < 0.001, ****p < 0.0001