Figure 5.

Hyperactivated T cells have an earlier and/or increased expression of CD25 and CD28. Human T cells and polymorphonuclear neutrophil granulocytes (PMN) were isolated from whole blood of healthy donors. PMN were incubated for 72 h in the presence or absence of nor-NOHA (1 mM). T cells were stimulated with anti-CD3/anti-CD28-tagged beads in the PMN-SN or in medium with or without the addition of 1 mM nor-NOHA for 24 h and 48 h. T cells were then harvested and stained with (A) anti-CD25, (B) anti-CD69, or (C) anti-CD28 antibodies and analyzed by flow cytometry. A summary of expression (MFI of CD3⁺ cells) of n=3 independent experiments with blood from different donors is demonstrated. Statistical calculations were performed with one-way ANOVA and Tukey´s post hoc test (***p < 0.001, **p < 0.01).