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. 2021 Feb 26;11:614082. doi: 10.3389/fonc.2021.614082

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Copyright © 2021 Zhu, Li, Bai, Shang, Fang, Cong, Li, Li, Song, Liu, Zhao, Li, Zhu and Jin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibitory effect of Apoptin on HepG-2 cells in vitro. Apoptin can significantly induce apoptosis, increase activity level and decrease mitochondrial membrane potential of Ad-Vp3-infected HepG-2 cells. (A) The results of inhibitory effect in Ad-Vp3-infected HepG-2 cells detected by Cell Counting Kit-8 and LDH ELISA Kit. (B) Hoechst staining (200×). (C) Annexin V-FITC/PI staining (200×). (D) Annexin V-FITC/PI flow cytometry detection. (E) Intracellular reactive oxygen species detection by ELISA. (F) JC-1 staining (200×). (G) JC-1 relative fluorescence detection. Data are presented as mean ± SD, *p < 0.05, **p < 0.01.