Fig. 4. Mtb forms biofilms inside the in vitro granulomas.
a Histopathology staining of 5 μm thick sections of uninfected and Mtb infected PBMCs placed on a collagen based 3D matrix visualized on a compound microscope. b CW and Auramine staining of the granuloma sections suggesting encapsulation of Mtb bacilli within a cellulose-rich extracellular matrix. All data are representative of three independent biological experiments. Scale bars correspond to 10 μm.