Skip to main content
. 2021 Mar 11;12:1606. doi: 10.1038/s41467-021-21748-6

Fig. 6. Mtb forms biofilms inside the lungs of non-human primates.

Fig. 6

a Histopathology of granulomatous lesions in the lungs of Mtb infected rhesus macaques in contrast to uninfected ones, as indicated by H & E staining. Clusters of acid-fast bacilli are indicated by arrows. b, c Cellulose staining with CW and corresponding quantitation (using NIS elements) of Mtb infected, uninfected and Cellulysin cellulase treated lung sections of rhesus macaques showing Mtb bacilli stained with Auramine B–Rhodamine O. d, e CBD-mCh staining for cellulose and subsequent quantification (using NIS elements) of Mtb infected, uninfected and Cellulysin cellulase treated lung sections of rhesus macaques showing Mtb bacilli (stained with Auramine B–Rhodamine O). f DNS assay of cellulase treated lung tissue blocks from uninfected and Mtb infected rhesus macaque. g Raman microscopy of (i) uninfected and (ii) Mtb infected rhesus macaque lung sections showing cellulose specific peaks in the infected lungs. The column bar graphs presented in figures c, e and f have been plotted in GraphPad Prism 6 and represented as mean (±s.e.m). Statistical significance was determined using Student’s t-test (two tailed). For c ****P < 0.0001, for e ****P < 0.0001 and for f ***P < 0.0001. All data are representative of three independent biological experiments performed in triplicates. Scale bars correspond to 50 μm. All source data are provided as a Source Data file.