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. 2021 Mar 11;12:1606. doi: 10.1038/s41467-021-21748-6

Fig. 7. Mtb forms biofilms in the human lungs.

Fig. 7

a H & E staining of human lung tissue sections. The clusters of acid-fast bacilli are denoted by arrows. b FISH with Mtb specific 16 S primers was utilized for identifying lung tissues infected with Mtb. c, d Cellulose was visualized using CW staining in Mtb positive and Mtb negative human lung tissue sections. Mtb bacilli were visualized using Auramine B–Rhodamine O staining. Mtb positive tissue sections were also stained with CW after Cellulysin Cellulase treatment. e, f CBD-mCh staining and visualization of Mtb (Auramine B–Rhodamine O) in Mtb positive and Mtb negative lung tissue sections. g DNS assay on Mtb positive and Mtb negative human lung tissue blocks after treatment with Cellulysin Cellulase. h Raman microscopy of (i) Mtb positive and (ii) Mtb negative human lung sections. The column bar graphs presented in figures d, f and g have been plotted in GraphPad Prism 6 and represented as mean (±s.e.m). Statistical significance was determined using Student’s t-test (two tailed). *P < 0.05, **P < 0.001, ***P < 0.0001. For d *P = 0.0169 (column A vs column B) and *P = 0.0401 (column B vs column C), for f ****P < 0.0001 and for g ***P = 0.0001 All data are representative of three independent biological experiments performed in triplicates. Scale bars correspond to 50 μm. All source data are provided as a Source Data file.