Table 2. Touchdown temperature protocol for Phusion U PCR .

Initial denaturation	Touchdown phase			Enrichment phase			Hold
	Denaturation	Annealing	Extension	Denaturation	Annealing	Extension
98 °C	98 °C	68 °C (-1 °C per cycle)	72 °C	98 °C	58 °C	72 °C	12 °C
30 s	10 s	15 s	20 s/kb	10 s	15 s	20 s/kb	∞
	10 cycles			27 cycles