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| Initial denaturation | Touchdown phase | Enrichment phase | Hold | ||||
|---|---|---|---|---|---|---|---|
| Denaturation | Annealing | Extension | Denaturation | Annealing | Extension | ||
| 94 °C | 94 °C |
62 °C
(–1 °C per cycle)
|
68 °C | 94 °C | 52 °C | 68 °C | 12 °C |
| 30 s | 20 s | 30 s | 1 min/kb | 20 s | 30 s | 1 min/kb | ∞ |
| 10 cycles | 27 cycles | ||||||
